Med12 Antibody

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NSJ Bioreagents
Product Code: NSJ-F54762
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54762-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54762-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the Med12 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Western blot testing of mouse NIH 3T3 cell lysate with Med12 antibody. Predicted molecular weight ~245 kDa.
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Western blot testing of mouse spleen tissue lysate with Med12 antibody. Predicted molecular weight ~245 kDa.
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Western blot testing of 1) human HeLa, 2) mouse C2C12 and 3) human Jurkat cell lysate with Med12 antibody. Predicted molecular weight ~245 kDa.
4 / 4
IHC testing of FFPE mouse stomach tissue with Med12 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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Western blot testing of mouse NIH 3T3 cell lysate with Med12 antibody. Predicted molecular weight ~245 kDa.
Western blot testing of mouse spleen tissue lysate with Med12 antibody. Predicted molecular weight ~245 kDa.
Western blot testing of 1) human HeLa, 2) mouse C2C12 and 3) human Jurkat cell lysate with Med12 antibody. Predicted molecular weight ~245 kDa.
IHC testing of FFPE mouse stomach tissue with Med12 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Documents

Further Information

Application Details :
Western blot: 1:1000-1:2000, Immunohistochemistry (FFPE): 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the Med12 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. This subunit may specifically regulate transcription of targets of the Wnt signaling pathway and SHH signaling pathway (By similarity).
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 1991-2025 from the mouse protein was used as the immunogen for the Med12 antibody.
Limitation:
This Med12 antibody is available for research use only.
Localization:
Nuclear
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse
Uniprot #:
A2AGH6