NUB1 Antibody / NEDD8 ultimate buster 1 / NYREN18

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NSJ Bioreagents
Product Code: NSJ-F54770
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54770-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54770-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the NYREN18 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Western blot testing of mouse liver lysate with NUB1 antibody. Expected molecular weight: 70-100 kDa depending on level of ubiquitination.
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Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with NUB1 antibody. Expected molecular weight: 70-100 kDa depending on level of ubiquitination.
3 / 3
IHC testing of FFPE human cancer tissue with NUB1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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Western blot testing of mouse liver lysate with NUB1 antibody. Expected molecular weight: 70-100 kDa depending on level of ubiquitination.
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with NUB1 antibody. Expected molecular weight: 70-100 kDa depending on level of ubiquitination.
IHC testing of FFPE human cancer tissue with NUB1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Documents

Further Information

Application Details :
Western blot: 1:500-1:1000, Immunohistochemistry (FFPE): 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the NYREN18 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
NUB1/NYREN18 is a specific down-regulator of the NEDD8 conjugation system. It recruits NEDD8 and its conjugates to the proteasome for degradation. It also interacts with PSMD4/S5a, a member of the regulatory subunit of the 26S proteasome.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 87-117 from the human protein was used as the immunogen for the NYREN18 antibody.
Limitation:
This NUB1 antibody is available for research use only.
Localization:
Nuclear, cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse
Uniprot #:
Q9Y5A7