PELP1 Antibody

NSJ Bioreagents
Product Code: NSJ-F54623
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54623-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54623-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the PELP1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Western blot testing of human T-47D, 2) mouse testis, 3) human HeLa, 4) human MCF7, 5) mouse NIH 3T3 and 6) human SW620 cell lysate with PELP1 antibody. Expected molecular weight: 120-160 kDa.
2 / 3
Western blot testing of mouse spleen tissue lysate with PELP1 antibody. Expected molecular weight: 120-160 kDa.
3 / 3
IHC testing of FFPE human colon tissue with PELP1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Western blot testing of human T-47D, 2) mouse testis, 3) human HeLa, 4) human MCF7, 5) mouse NIH 3T3 and 6) human SW620 cell lysate with PELP1 antibody. Expected molecular weight: 120-160 kDa.
Western blot testing of mouse spleen tissue lysate with PELP1 antibody. Expected molecular weight: 120-160 kDa.
IHC testing of FFPE human colon tissue with PELP1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Further Information

Application Details :
Western blot: 1:500-1:2000, Immunohistochemistry (FFPE): 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the PELP1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Proline-, glutamic acid- and leucine-rich protein 1 is a coactivator of estrogen receptor-mediated transcription and a corepressor of other nuclear hormone receptors and sequence-specific transcription factors.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 744-771 from the human protein was used as the immunogen for the PELP1 antibody.
Limitation:
This PELP1 antibody is available for research use only.
Localization:
Cytoplasmic, nuclear
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse
Uniprot #:
Q8IZL8