SFRP5 Antibody

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NSJ Bioreagents
Product Code: NSJ-F54368
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54368-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54368-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the SFRP5 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 3
IHC testing of FFPE human pancreas tissue with SFRP5 antibody. HIER: steam section in pH9 EDTA for 20 min and allow to cool prior to staining.
2 / 3
IHC testing of FFPE human colon tissue with SFRP5 antibody. HIER: steam section in pH9 EDTA for 20 min and allow to cool prior to staining.
3 / 3
Western blot testing of 1) human liver, 2) human pancreas, 3) human HeLa, 4) mouse eyeball and 5) mouse pancreas lysate with SFRP5 antibody. Predicted molecular weight ~36 kDa.
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IHC testing of FFPE human pancreas tissue with SFRP5 antibody. HIER: steam section in pH9 EDTA for 20 min and allow to cool prior to staining.
IHC testing of FFPE human colon tissue with SFRP5 antibody. HIER: steam section in pH9 EDTA for 20 min and allow to cool prior to staining.
Western blot testing of 1) human liver, 2) human pancreas, 3) human HeLa, 4) mouse eyeball and 5) mouse pancreas lysate with SFRP5 antibody. Predicted molecular weight ~36 kDa.

Documents

Further Information

Application Details :
Western blot: 1:500-1:2000,Immunohistochemistry (FFPE): 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the SFRP5 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Secreted frizzled-related protein 5 (SFRP5) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. SFRP5 and SFRP1 may be involved in determining the polarity of photoreceptor cells in the retina. SFRP5 is highly expressed in the retinal pigment epithelium, and moderately expressed in the pancreas. [provided by RefSeq].
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 190-219 from the human protein were used as the immunogen for the SFRP5 antibody.
Limitation:
This SFRP5 antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse
Uniprot #:
Q5T4F7