SAE1 Antibody

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NSJ Bioreagents
Product Code: NSJ-F54771
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54771-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54771-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the SUMO-activating enzyme subunit 1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Western blot testing of human HEK293 cell lysate with SUMO-activating enzyme subunit 1 antibody. Predicted molecular weight ~38 kDa.
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Western blot testing of mouse heart tissue lysate with SUMO-activating enzyme subunit 1 antibody. Predicted molecular weight ~38 kDa.
3 / 3
IHC testing of FFPE human breast cancer tissue with SUMO-activating enzyme subunit 1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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Western blot testing of human HEK293 cell lysate with SUMO-activating enzyme subunit 1 antibody. Predicted molecular weight ~38 kDa.
Western blot testing of mouse heart tissue lysate with SUMO-activating enzyme subunit 1 antibody. Predicted molecular weight ~38 kDa.
IHC testing of FFPE human breast cancer tissue with SUMO-activating enzyme subunit 1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Documents

Further Information

Application Details :
Western blot: 1:500-1:1000, Immunohistochemistry (FFPE): 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the SUMO-activating enzyme subunit 1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
The dimeric enzyme AOS1 acts as a an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins and formation of a thioester with a conserved cysteine residue on SAE2.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 300-329 from the human protein was used as the immunogen for the SUMO-activating enzyme subunit 1 antibody.
Limitation:
This SUMO-activating enzyme subunit 1 antibody is available for research use only.
Localization:
Nuclear, cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse
Uniprot #:
Q9UBE0