UBE1C Antibody / UBA3

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NSJ Bioreagents
Product Code: NSJ-F54550
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54550-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54550-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the UBA3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Western blot testing of human HL60 cell lysate with UBA3 antibody. Expected molecular weight ~52 kDa.
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Western blot testing of mouse brain lysate with UBA3 antibody. Expected molecular weight ~52 kDa.
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Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with UBA3 antibody. Predicted molecular weight ~52 kDa.
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IHC testing of FFPE human cancer tissue with UBA3 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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Western blot testing of human HL60 cell lysate with UBA3 antibody. Expected molecular weight ~52 kDa.
Western blot testing of mouse brain lysate with UBA3 antibody. Expected molecular weight ~52 kDa.
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with UBA3 antibody. Predicted molecular weight ~52 kDa.
IHC testing of FFPE human cancer tissue with UBA3 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Documents

Further Information

Application Details :
Western blot: 1:500-1:2000, Immunohistochemistry (FFPE): 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the UBA3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E1 ubiquitin-activating enzyme family. The encoded enzyme associates with AppBp1, an amyloid beta precursor protein binding protein, to form a heterodimer, and then the enzyme complex activates NEDD8, a ubiquitin-like protein, which regulates cell division, signaling and embryogenesis. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 415-445 from the human protein was used as the immunogen for the UBA3 antibody.
Limitation:
This UBA3 antibody is available for research use only.
Localization:
Nuclear, cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse
Uniprot #:
Q8TBC4