UBE4B Antibody

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NSJ Bioreagents
Product Code: NSJ-F54394
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54394-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54394-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the UBE4B antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 3
Western blot testing of human 1) K562, 2) Raji, 3) HeLa and 4) NIH 3T3 lysate with UBE4B antibody. Predicted molecular weight ~146 kDa.
2 / 3
IHC testing of FFPE human pancreas tissue with UBE4B antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
3 / 3
IHC testing of FFPE human testis tissue with UBE4B antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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Western blot testing of human 1) K562, 2) Raji, 3) HeLa and 4) NIH 3T3 lysate with UBE4B antibody. Predicted molecular weight ~146 kDa.
IHC testing of FFPE human pancreas tissue with UBE4B antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE human testis tissue with UBE4B antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Documents

Further Information

Application Details :
Western blot: 1:500-1:2000,Immunohistochemistry (FFPE): 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the UBE4B antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE4B represents an additional conjugation factor, E4, which is involved in multiubiquitin chain assembly. UBE4B also the strongest candidate among the neuroblastoma tumor suppressors.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 112-142 from the human protein was used as the immunogen for the UBE4B antibody.
Limitation:
This UBE4B antibody is available for research use only.
Localization:
Nuclear, cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse
Uniprot #:
O95155