Angiopoietin 2 Antibody / ANG2

NSJ Bioreagents
Product Code: NSJ-F54532
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54532-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54532-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the Angiopoietin 2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
IHC testing of FFPE human uterus tissue with Angiopoietin 2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 4
Western blot testing of human liver lysate with Angiopoietin 2 antibody. Predicted molecular weight ~57 kDa.
3 / 4
Western blot testing of mouse kidney lysate with Angiopoietin 2 antibody. Predicted molecular weight ~57 kDa.
4 / 4
Flow cytometry testing of fixed and permeabilized human A549 cells with Angiopoietin 2 antibody; Blue=isotype control, Green= Angiopoietin 2 antibody.

IHC testing of FFPE human uterus tissue with Angiopoietin 2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of human liver lysate with Angiopoietin 2 antibody. Predicted molecular weight ~57 kDa.
Western blot testing of mouse kidney lysate with Angiopoietin 2 antibody. Predicted molecular weight ~57 kDa.
Flow cytometry testing of fixed and permeabilized human A549 cells with Angiopoietin 2 antibody; Blue=isotype control, Green= Angiopoietin 2 antibody.

Further Information

Application Details :
Western blot: 1:500-1:2000, Immunohistochemistry (FFPE): 1:25, Flow cytometry: 1:25 (1x10e6 cells)
Application Note:
The stated application concentrations are suggested starting points. Titration of the Angiopoietin 2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
The protein encoded by this gene is an antagonist of angiopoietin 1 (ANGPT1) and endothelial TEK tyrosine kinase (TIE-2, TEK). The encoded protein disrupts the vascular remodeling ability of ANGPT1 and may induce endothelial cell apoptosis. Three transcript variants encoding three different isoforms have been found for this gene.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 404-432 from the human protein was used as the immunogen for the Angiopoietin 2 antibody.
Limitation:
This Angiopoietin 2 antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse
Uniprot #:
O15123