ELAVL2 Antibody / ELAV-like protein 2

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NSJ Bioreagents
Product Code: NSJ-F54281
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54281-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54281-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the ELAVL2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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IHC testing of FFPE human brain tissue with ELAVL2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 5
IHC testing of FFPE human cerebellum tissue with ELAVL2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
3 / 5
Western blot testing of human brain lysate with ELAVL2 antibody. Predicted molecular weight ~40 kDa.
4 / 5
Western blot testing of mouse brain lysate with ELAVL2 antibody. Predicted molecular weight ~40 kDa.
5 / 5
Flow cytometry testing of fixed and permeabilized human K562 cells with ELAVL2 antibody; Blue=isotype control, Green= ELAVL2 antibody.
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IHC testing of FFPE human brain tissue with ELAVL2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE human cerebellum tissue with ELAVL2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of human brain lysate with ELAVL2 antibody. Predicted molecular weight ~40 kDa.
Western blot testing of mouse brain lysate with ELAVL2 antibody. Predicted molecular weight ~40 kDa.
Flow cytometry testing of fixed and permeabilized human K562 cells with ELAVL2 antibody; Blue=isotype control, Green= ELAVL2 antibody.

Documents

Further Information

Application Details :
Western blot: 1:500-1:2000,Immunohistochemistry (FFPE): 1:25,Flow cytometry: 1:25 (1x10e6 cells)
Application Note:
The stated application concentrations are suggested starting points. Titration of the ELAVL2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
The protein encoded by this gene is a neural-specific RNA-binding protein that is known to bind to several 3' UTRs, including its own and also that of FOS and ID. The encoded protein may recognize a GAAA motif in the RNA. Three transcript variants encoding two different isoforms have been found for this gene.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 156-184 from the human protein were used as the immunogen for the ELAVL2 antibody.
Limitation:
This ELAVL2 antibody is available for research use only.
Localization:
Nuclear, cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse
Uniprot #:
Q12926