ZBP-1 Antibody / IGF2BP1

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NSJ Bioreagents
Product Code: NSJ-F54545
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54545-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54545-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the IGF2BP1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
IHC testing of FFPE human testis tissue with IGF2BP1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 4
IHC testing of FFPE human lung adenocarcinoma tissue with IGF2BP1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
3 / 4
Western blot testing of human 1) HEK293, 2) RD, 3) Karpas 299, 4) Raji, 5) Jurkat, 6) HeLa, 7) HepG2 and 8) mouse NIH 3T3 cell lysate with IGF2BP1 antibody. Predicted molecular weight ~64 kDa.
4 / 4
Flow cytometry testing of fixed and permeabilized human HepG2 cells with IGF2BP1 antibody; Blue=isotype control, Green= IGF2BP1 antibody.
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IHC testing of FFPE human testis tissue with IGF2BP1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE human lung adenocarcinoma tissue with IGF2BP1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of human 1) HEK293, 2) RD, 3) Karpas 299, 4) Raji, 5) Jurkat, 6) HeLa, 7) HepG2 and 8) mouse NIH 3T3 cell lysate with IGF2BP1 antibody. Predicted molecular weight ~64 kDa.
Flow cytometry testing of fixed and permeabilized human HepG2 cells with IGF2BP1 antibody; Blue=isotype control, Green= IGF2BP1 antibody.

Documents

Further Information

Application Details :
Western blot: 1:500-1:2000, Immunohistochemistry (FFPE): 1:25, Flow cytometry: 1:25 (1x10e6 cells)
Application Note:
The stated application concentrations are suggested starting points. Titration of the IGF2BP1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
IGF2BP1 is a member of the insulin-like growth factor 2 mRNA-binding protein family. The protein encoded by this gene contains four K homology domains and two RNA recognition motifs. It functions by binding to the mRNAs of certain genes, including insulin-like growth factor 2, beta-actin and beta-transducin repeat-containing protein, and regulating their translation. Two transcript variants encoding different isoforms have been found for this gene.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 508-534 from the human protein was used as the immunogen for the IGF2BP1 antibody.
Limitation:
This IGF2BP1 antibody is available for research use only.
Localization:
Cytoplasmic, nuclear
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse
Uniprot #:
Q9NZI8