MYL1 Antibody (Center Region)

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NSJ Bioreagents
Product Code: NSJ-F54184
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54184-0.05ML0.05 ml£260.00
Quantity:
NSJ-F54184-0.2ML0.2 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the MYL1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 3
Western blot testing of 1) human heart, 2) human skeletal muscle, 3) mouse skeletal muscle and 4) rat skeletal muscle lysate with MYL1 antibody at 1:2000. Predicted molecular weight ~21 kDa.
2 / 3
IHC staining of FFPE human heart with MYL1 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
3 / 3
Flow cytometry testing of fixed and permeabilized human HeLa cells with MYL1 antibody (green) and isotype control (blue).
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Western blot testing of 1) human heart, 2) human skeletal muscle, 3) mouse skeletal muscle and 4) rat skeletal muscle lysate with MYL1 antibody at 1:2000. Predicted molecular weight ~21 kDa.
IHC staining of FFPE human heart with MYL1 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
Flow cytometry testing of fixed and permeabilized human HeLa cells with MYL1 antibody (green) and isotype control (blue).

Documents

Further Information

Application Details :
Western blot: 1:1000-1:2000,Immunohistochemistry (FFPE): 1:25,Flow cytometry: 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the MYL1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Myosin light chain 1/3, skeletal muscle isoform, is the regulatory light chain of myosin. Does not bind calcium.
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 101-135 from human MYL1 was used as the immunogen for the MYL1 antibody.
Limitation:
This MYL1 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse
Uniprot #:
P05976