Atrial Natriuretic Peptide/Factor Antibody / ANP / ANF

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NSJ Bioreagents
Product Code: NSJ-F54517
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54517-0.05ML0.05 ml£260.00
Quantity:
NSJ-F54517-0.2ML0.2 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the NPPA antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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IHC testing of FFPE human prostate carcinoma tissue with NPPA antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 3
Flow cytometry testing of fixed and permeabilized human RD cells with NPPA antibody; Blue=isotype control, Green = NPPA antibody.
3 / 3
Western blot testing of 1) human fetal heart and 2) mouse heart lysate with NPPA antibody. Predicted molecular weight ~17 kDa.
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IHC testing of FFPE human prostate carcinoma tissue with NPPA antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Flow cytometry testing of fixed and permeabilized human RD cells with NPPA antibody; Blue=isotype control, Green = NPPA antibody.
Western blot testing of 1) human fetal heart and 2) mouse heart lysate with NPPA antibody. Predicted molecular weight ~17 kDa.

Documents

Further Information

Application Details :
Western blot: 1:500-1:2000, Immunohistochemistry (FFPE): 1:25, Flow cytometry: 1:25 (1x10e6 cells)
Application Note:
The stated application concentrations are suggested starting points. Titration of the NPPA antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
NPPA belongs to the natriuretic peptide family. Natriuretic peptides are implicated in the control of extracellular fluid volume and electrolyte homeostasis. This protein is synthesized as a large precursor(containing a signal peptide), which is processed to release a peptide from the N-terminus with similarity to vasoactive peptide, cardiodilatin, and another peptide from the C-terminus with natriuretic-diuretic activity.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 30-56 from the human protein was used as the immunogen for the NPPA antibody.
Limitation:
This NPPA antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse
Uniprot #:
P01160