PARS2 Antibody / Prolyl-tRNA synthetase / ProRS

NSJ Bioreagents
Product Code: NSJ-F54574
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54574-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54574-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the Prolyl-tRNA synthetase antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
IHC testing of FFPE human kidney tissue with Prolyl-tRNA synthetase antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 4
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with Prolyl-tRNA synthetase antibody. Predicted molecular weight ~53 kDa.
3 / 4
Western blot testing of mouse bladder tissue lysate with Prolyl-tRNA synthetase antibody. Predicted molecular weight ~53 kDa.
4 / 4
Flow cytometry testing of human HEK293 cells with Prolyl-tRNA synthetase antibody; Blue=isotype control, Green= Prolyl-tRNA synthetase antibody.

IHC testing of FFPE human kidney tissue with Prolyl-tRNA synthetase antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with Prolyl-tRNA synthetase antibody. Predicted molecular weight ~53 kDa.
Western blot testing of mouse bladder tissue lysate with Prolyl-tRNA synthetase antibody. Predicted molecular weight ~53 kDa.
Flow cytometry testing of human HEK293 cells with Prolyl-tRNA synthetase antibody; Blue=isotype control, Green= Prolyl-tRNA synthetase antibody.

Further Information

Application Details :
Western blot: 1:500-1:2000, Immunohistochemistry (FFPE): 1:25, Flow cytometry: 1:25 (1x10e6 cells)
Application Note:
The stated application concentrations are suggested starting points. Titration of the Prolyl-tRNA synthetase antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Forms part of a macromolecular complex that catalyzes the attachment of specific amino acids to cognate tRNAs during protein synthesis. Modulates the secretion of AIMP1 and may be involved in generation of the inflammatory cytokine EMAP2 from AIMP1.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 390-417 from the human protein was used as the immunogen for the Prolyl-tRNA synthetase antibody.
Limitation:
This Prolyl-tRNA synthetase antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Purified
Species Reactivity :
Human, Mouse
Uniprot #:
Q7L3T8