Ufm1-conjugating enzyme 1 Antibody / Ufc1

NSJ Bioreagents
Product Code: NSJ-F54767
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54767-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54767-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the Ufm1-conjugating enzyme 1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
IHC testing of FFPE human bladder carcinoma tissue with Ufm1-conjugating enzyme 1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 4
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with Ufm1-conjugating enzyme 1 antibody.
3 / 4
Western blot testing of mouse kidney tissue lysate with Ufm1-conjugating enzyme 1 antibody. Predicted molecular weight ~19 kDa.
4 / 4
Flow cytometry testing of human HEK293 cells with Ufm1-conjugating enzyme 1 antibody; Blue=isotype control, Green= Ufm1-conjugating enzyme 1 antibody.

IHC testing of FFPE human bladder carcinoma tissue with Ufm1-conjugating enzyme 1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with Ufm1-conjugating enzyme 1 antibody.
Western blot testing of mouse kidney tissue lysate with Ufm1-conjugating enzyme 1 antibody. Predicted molecular weight ~19 kDa.
Flow cytometry testing of human HEK293 cells with Ufm1-conjugating enzyme 1 antibody; Blue=isotype control, Green= Ufm1-conjugating enzyme 1 antibody.

Further Information

Application Details :
Western blot: 1:500-1:1000, Immunohistochemistry (FFPE): 1:10-1:50, Flow cytometry: 1:10-1:50 (1x10e6 cells)
Application Note:
The stated application concentrations are suggested starting points. Titration of the Ufm1-conjugating enzyme 1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Various ubiquitin-like (UBL) post-translational modifiers contribute critically to the protein degradation pathway . These UBLs are covalently linked to most target proteins through an enzymatic cascade analogous to ubiquitylation, consisting of E1 (activating), E2 (conjugating), and E3 (ligating) enzymes. Ufc1 Belongs to the ubiquitin-conjugating enzyme family, and is an E2-like enzyme which forms an intermediate with UFM1, a novel ubiquitin-fold modifier, via thioester linkage.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
An amino acid sequence from the C-terminal region of the human protein was used as the immunogen for the Ufm1-conjugating enzyme 1 antibody.
Limitation:
This Ufm1-conjugating enzyme 1 antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse
Uniprot #:
Q9Y3C8