Villin Antibody

Contact us
NSJ Bioreagents
Product Code: NSJ-F54302
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54302-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54302-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the VIL1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 6
Western blot testing of 1) human HT-29, 2) human HepG2, 3) human COLO205 and 4) mouse colon lysate with VIL1 antibody. Predicted molecular weight ~93 kDa.
2 / 6
Western blot testing of 1) human HepG2, 2) human HT-29 and 3) mouse kidney lysate with VIL1 antibody. Predicted molecular weight ~93 kDa.
3 / 6
Western blot testing of human 1) Caco-2 and 2) HT-29 cell lysate with VIL1 antibody. Predicted molecular weight ~93 kDa.
4 / 6
Western blot testing of 1) human HT-29, 2) human SW480 and 3) mouse colon lysate with VIL1 antibody. Predicted molecular weight ~93 kDa.
5 / 6
IHC testing of FFPE human kidney tissue with VIL1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
6 / 6
Flow cytometry testing of fixed and permeabilized human HeLa cells with VIL1 antibody; Blue=isotype control, Green= VIL1 antibody.
❮❯

Western blot testing of 1) human HT-29, 2) human HepG2, 3) human COLO205 and 4) mouse colon lysate with VIL1 antibody. Predicted molecular weight ~93 kDa.
Western blot testing of 1) human HepG2, 2) human HT-29 and 3) mouse kidney lysate with VIL1 antibody. Predicted molecular weight ~93 kDa.
Western blot testing of human 1) Caco-2 and 2) HT-29 cell lysate with VIL1 antibody. Predicted molecular weight ~93 kDa.
Western blot testing of 1) human HT-29, 2) human SW480 and 3) mouse colon lysate with VIL1 antibody. Predicted molecular weight ~93 kDa.
IHC testing of FFPE human kidney tissue with VIL1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Flow cytometry testing of fixed and permeabilized human HeLa cells with VIL1 antibody; Blue=isotype control, Green= VIL1 antibody.

Documents

Further Information

Application Details :
Western blot: 1:500-1:2000,Immunohistochemistry (FFPE): 1:25,Flow cytometry: 1:25 (1x10e6 cells)
Application Note:
The stated application concentrations are suggested starting points. Titration of the VIL1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Villin-1 is a member of a family of calcium-regulated actin-binding proteins. This protein represents a dominant part of the brush border cytoskeleton which functions in the capping, severing, and bundling of actin filaments.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 180-207 from the human protein were used as the immunogen for the VIL1 antibody.
Limitation:
This VIL1 antibody is available for research use only.
Localization:
Cytoplasmic and cell surface
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse
Uniprot #:
P09327