DHCR7 Antibody

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NSJ Bioreagents
Product Code: NSJ-F54352
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54352-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54352-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the DHCR7 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 5
Western blot testing of human 1) HepG2, 2) Li-7, 3) U-87 MG, 4) testis and 5) mouse testis lysate with DHCR7 antibody. Predicted molecular weight ~54 kDa.
2 / 5
Western blot testing of mouse liver lysate with DHCR7 antibody. Predicted molecular weight ~54 kDa.
3 / 5
Immunofluorescent staining of fixed and permeabilized human HeLa cells with DHCR7 antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
4 / 5
Immunofluorescent staining of fixed and permeabilized human MCF7 cells with DHCR7 antibody (green) and DAPI nuclear stain (blue).
5 / 5
IHC testing of FFPE human testis tissue with DHCR7 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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Western blot testing of human 1) HepG2, 2) Li-7, 3) U-87 MG, 4) testis and 5) mouse testis lysate with DHCR7 antibody. Predicted molecular weight ~54 kDa.
Western blot testing of mouse liver lysate with DHCR7 antibody. Predicted molecular weight ~54 kDa.
Immunofluorescent staining of fixed and permeabilized human HeLa cells with DHCR7 antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
Immunofluorescent staining of fixed and permeabilized human MCF7 cells with DHCR7 antibody (green) and DAPI nuclear stain (blue).
IHC testing of FFPE human testis tissue with DHCR7 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Documents

Further Information

Application Details :
Western blot: 1:500-1:2000,Immunohistochemistry (FFPE): 1:25,Immunofluorescence: 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the DHCR7 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This gene encodes an enzyme that removes the C(7-8) double bond in the B ring of sterols and catalyzes the conversion of 7-dehydrocholesterol to cholesterol. This gene is ubiquitously expressed and its transmembrane protein localizes to the endoplasmic reticulum membrane and nuclear outer membrane. Mutations in this gene cause Smith-Lemli-Opitz syndrome (SLOS); a syndrome that is metabolically characterized by reduced serum cholesterol levels and elevated serum 7-dehydrocholesterol levels and phenotypically characterized by mental retardation, facial dysmorphism, syndactyly of second and third toes, and holoprosencephaly in severe cases to minimal physical abnormalities and near-normal intelligence in mild cases. Alternative splicing results in multiple transcript variants that encode the same protein.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 437-463 from the human protein was used as the immunogen for the DHCR7 antibody.
Limitation:
This DHCR7 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse
Uniprot #:
Q9UBM7