HSP60 Antibody

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NSJ Bioreagents
Product Code: NSJ-F54325
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54325-0.05ML0.05 ml£260.00
Quantity:
NSJ-F54325-0.2ML0.2 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the Hsp60 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
Western blot testing of human 1) HeLa, 2) MCF7, 3) HepG2, 4) Jurkat, 5) mouse NIH 3T3 and 6) mouse thymus lysate with Hsp60 antibody. Predicted molecular weight: ~60 kDa.
2 / 4
IHC testing of FFPE human lung adenocarcinoma tissue with Hsp60 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
3 / 4
IHC testing of FFPE human kidney tissue with Hsp60 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
4 / 4
Immunofluorescent staining of fixed and permeabilized human HepG2 cells with Hsp60 antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
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Western blot testing of human 1) HeLa, 2) MCF7, 3) HepG2, 4) Jurkat, 5) mouse NIH 3T3 and 6) mouse thymus lysate with Hsp60 antibody. Predicted molecular weight: ~60 kDa.
IHC testing of FFPE human lung adenocarcinoma tissue with Hsp60 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE human kidney tissue with Hsp60 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of fixed and permeabilized human HepG2 cells with Hsp60 antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).

Documents

Further Information

Application Details :
Western blot: 1:500-1:2000,Immunohistochemistry (FFPE): 1:25,Immunofluorescence: 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the Hsp60 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 340-374 from the human protein was used as the immunogen for the Hsp60 antibody.
Limitation:
This Hsp60 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse
Uniprot #:
P10809