IFITM3 Antibody

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NSJ Bioreagents
Product Code: NSJ-F54274
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54274-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54274-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the IFITM3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 5
Western blot testing of human 1) HeLa, 2) 293, 3) A549, 4) NCI-H460, 5) HepG2, 6) SK-B-R3 and 7) A375 cell lysate with IFITM3 antibody. Predicted molecular weight: ~15/17 kDa (unmodified/lipidated).
2 / 5
Western blot testing of human 1) HeLa, 2) 293, 3) HepG2, 4) NCI-H460, 5) SK-B-R3, 6) placent and 7) mouse colon lysate with IFITM3 antibody. Predicted molecular weight: ~15/17 kDa (unmodified/lipidated).
3 / 5
IHC testing of FFPE human spleen tissue with IFITM3 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
4 / 5
IHC testing of FFPE human colon carcinoma tissue with IFITM3 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
5 / 5
Immunofluorescent staining of fixed and permeabilized human HeLa cells with IFITM3 antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
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Western blot testing of human 1) HeLa, 2) 293, 3) A549, 4) NCI-H460, 5) HepG2, 6) SK-B-R3 and 7) A375 cell lysate with IFITM3 antibody. Predicted molecular weight: ~15/17 kDa (unmodified/lipidated).
Western blot testing of human 1) HeLa, 2) 293, 3) HepG2, 4) NCI-H460, 5) SK-B-R3, 6) placent and 7) mouse colon lysate with IFITM3 antibody. Predicted molecular weight: ~15/17 kDa (unmodified/lipidated).
IHC testing of FFPE human spleen tissue with IFITM3 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE human colon carcinoma tissue with IFITM3 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of fixed and permeabilized human HeLa cells with IFITM3 antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).

Documents

Further Information

Application Details :
Western blot: 1:500-1:2000,Immunohistochemistry (FFPE): 1:25,Immunofluorescence: 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the IFITM3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
The family of interferon-induced transmembrane protein (Ifitm/mil/fragilis) cell surface proteins may modulate cell adhesion and influence cell differentiation.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 1-30 from the human protein were used as the immunogen for the IFITM3 protein Antibody.
Limitation:
This IFITM3 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse
Uniprot #:
Q01628