Neurogenin 3 Antibody / NGN3

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NSJ Bioreagents
Product Code: NSJ-F54290
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54290-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54290-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the NGN3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 6
Western blot testing of human placenta lysate with NGN3 antibody. Predicted molecular weight ~23 kDa.
2 / 6
Western blot testing of human ThP-1 cell lysate with NGN3 antibody. Predicted molecular weight ~23 kDa.
3 / 6
Western blot testing of human 1) DU145, 2) LNCaP, 3) PANC1 and 4) PC-3 cell lysate with NGN3 antibody. Predicted molecular weight ~23 kDa.
4 / 6
Immunofluorescent staining of fixed and permeabilized human NCI-H460 cells with NGN3 antibody (green) and DAPI nuclear stain (blue).
5 / 6
IHC testing of FFPE human small intestine tissue with NGN3 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
6 / 6
IHC testing of FFPE human pancreas tissue with NGN3 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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Western blot testing of human placenta lysate with NGN3 antibody. Predicted molecular weight ~23 kDa.
Western blot testing of human ThP-1 cell lysate with NGN3 antibody. Predicted molecular weight ~23 kDa.
Western blot testing of human 1) DU145, 2) LNCaP, 3) PANC1 and 4) PC-3 cell lysate with NGN3 antibody. Predicted molecular weight ~23 kDa.
Immunofluorescent staining of fixed and permeabilized human NCI-H460 cells with NGN3 antibody (green) and DAPI nuclear stain (blue).
IHC testing of FFPE human small intestine tissue with NGN3 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE human pancreas tissue with NGN3 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Documents

Further Information

Application Details :
Western blot: 1:500-1:2000,Immunohistochemistry (FFPE): 1:25,Immunofluorescence: 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the NGN3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Neurogenin-3 belongs to a family of basic helix-loop-helix transcription factors involved in the determination of neural precursor cells in the neuroectoderm. Neurogenin-3 also required for the specification of a common precursor of the 4 pancreatic endocrine cell types. Defects in NEUROG3 are the cause of congenital malabsorptive diarrhea 4 (DIAR4). DIAR4 is an autosomal recessive disorder characterized by generalized malabsorption and a paucity of enteroendocrine cells.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 40-69 from the human protein were used as the immunogen for the NGN3 antibody.
Limitation:
This NGN3 antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse
Uniprot #:
Q9Y4Z2