Alpha Enolase Antibody / ENOA

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NSJ Bioreagents
Product Code: NSJ-F54785
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54785-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54785-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the Alpha Enolase antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
Western blot testing of 1) human Jurkat, 2) human MCF7, 3) human U-251 and 4) mouse brain lysate with Alpha Enolase antibody. Predicted molecular weight ~47 kDa.
2 / 4
IHC testing of FFPE human kidney tissue with Alpha Enolase antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
3 / 4
Immunofluorescent staining of human HeLa cells with Alpha Enolase antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
4 / 4
Flow cytometry testing of human Ramos cells with Alpha Enolase antibody; Blue=isotype control, Green= Alpha Enolase antibody.
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Western blot testing of 1) human Jurkat, 2) human MCF7, 3) human U-251 and 4) mouse brain lysate with Alpha Enolase antibody. Predicted molecular weight ~47 kDa.
IHC testing of FFPE human kidney tissue with Alpha Enolase antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of human HeLa cells with Alpha Enolase antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
Flow cytometry testing of human Ramos cells with Alpha Enolase antibody; Blue=isotype control, Green= Alpha Enolase antibody.

Documents

Further Information

Application Details :
Western blot: 1:500-1:1000, Immunohistochemistry (FFPE): 1:25, Immunofluorescence: 1:25, Flow cytometry: 1:25 (1x10e6 cells)
Application Note:
The stated application concentrations are suggested starting points. Titration of the Alpha Enolase antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
ENOA, or ENO1, is one of three enolase isoenzymes found in mammals; the protein alpha-enolase, a homodimeric soluble enzyme, and is also a shorter monomeric structural lens protein, tau-crystallin. The two proteins are made from the same message. The full length protein, the isoenzyme, is found in the cytoplasm. The shorter protein is produced from an alternative translation start, is localized to the nucleus, and has been found to bind to an element in the c-myc promoter.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 405-434 from the human protein was used as the immunogen for the Alpha Enolase antibody.
Limitation:
This Alpha Enolase antibody is available for research use only.
Localization:
Cytoplasmic, nuclear
Purity:
Purified
Species Reactivity :
Human, Mouse
Uniprot #:
P06733