GRP78 Antibody / BiP / HSPA5

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NSJ Bioreagents
Product Code: NSJ-F54266
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54266-0.05ML0.05 ml£260.00
Quantity:
NSJ-F54266-0.2ML0.2 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the HSPA5 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Western blot testing of human HL60 cell lysate with HSPA5 antibody. Predicted molecular weight: ~73 kDa, but routinely observed at 70-78 kDa.
2 / 6
Western blot testing of mouse liver cell lysate with HSPA5 antibody. Predicted molecular weight: ~73 kDa, but routinely observed at 70-78 kDa.
3 / 6
Immunofluorescent staining of fixed and permeabilized human NCI-H460 cells with HSPA5 antibody (green) and DAPI nuclear stain (blue).
4 / 6
IHC testing of FFPE human testis tissue with HSPA5 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
5 / 6
IHC testing of FFPE human prostate carcinoma with HSPA5 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
6 / 6
Flow cytometry testing of fixed and permeabilized human HeLa cells with HSPA5 antibody; Blue=isotype control, Green= HSPA5 antibody.
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Western blot testing of human HL60 cell lysate with HSPA5 antibody. Predicted molecular weight: ~73 kDa, but routinely observed at 70-78 kDa.
Western blot testing of mouse liver cell lysate with HSPA5 antibody. Predicted molecular weight: ~73 kDa, but routinely observed at 70-78 kDa.
Immunofluorescent staining of fixed and permeabilized human NCI-H460 cells with HSPA5 antibody (green) and DAPI nuclear stain (blue).
IHC testing of FFPE human testis tissue with HSPA5 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE human prostate carcinoma with HSPA5 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Flow cytometry testing of fixed and permeabilized human HeLa cells with HSPA5 antibody; Blue=isotype control, Green= HSPA5 antibody.

Documents

Further Information

Application Details :
Western blot: 1:500-1:2000,Immunohistochemistry (FFPE): 1:25,Immunofluorescence: 1:25,Flow cytometry: 1:25 (1x10e6 cells)
Application Note:
The stated application concentrations are suggested starting points. Titration of the HSPA5 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
In cooperation with other chaperones, HSP70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. The HSP70s in mitochondria and the endoplasmic reticulum play an additional role by providing a driving force for protein translocation. They are involved in signal transduction pathways in cooperation with HSP90. They participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
Full length recombinant human protein was used as the immunogen for the HSPA5 Antibody.
Limitation:
This HSPA5 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
SAS precipitation
Species Reactivity :
Human, Mouse
Uniprot #:
P11021