STRADA Antibody / LYK5

Contact us
NSJ Bioreagents
Product Code: NSJ-F54391
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54391-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54391-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the STRADA antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 5
Immunofluorescent staining of human A549 cells with STRADA antibody (green) and anti-Actin (red).
2 / 5
IHC testing of FFPE human brain tissue with STRADA antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
3 / 5
Western blot testing of 1) human 293, 2) human MOLT-4 and 3) mouse brain lysate with STRADA antibody. Predicted molecular weight ~48 kDa.
4 / 5
Western blot testing of 1) human 293, 2) human HepG2, 3) human MOLT-4, 4) mouse NIH 3T3 and 5) mouse brain lysate with STRADA antibody. Predicted molecular weight ~48 kDa.
5 / 5
Flow cytometry testing of human SH-SY5Y cells with STRADA antibody; Blue=isotype control, Green= STRADA antibody.
❮❯

Immunofluorescent staining of human A549 cells with STRADA antibody (green) and anti-Actin (red).
IHC testing of FFPE human brain tissue with STRADA antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of 1) human 293, 2) human MOLT-4 and 3) mouse brain lysate with STRADA antibody. Predicted molecular weight ~48 kDa.
Western blot testing of 1) human 293, 2) human HepG2, 3) human MOLT-4, 4) mouse NIH 3T3 and 5) mouse brain lysate with STRADA antibody. Predicted molecular weight ~48 kDa.
Flow cytometry testing of human SH-SY5Y cells with STRADA antibody; Blue=isotype control, Green= STRADA antibody.

Documents

Further Information

Application Details :
Western blot: 1:500-1:2000,Immunohistochemistry (FFPE): 1:25,Immunofluorescence: 1:25,Flow cytometry: 1:25 (1x10e6 cells)
Application Note:
The stated application concentrations are suggested starting points. Titration of the STRADA antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Pseudokinase which, in complex with CAB39/MO25 (CAB39/MO25alpha or CAB39L/MO25beta), binds to and activates STK11/LKB1. Adopts a closed conformation typical of active protein kinases and binds STK11/LKB1 as a pseudosubstrate, promoting conformational change of STK11/LKB1 in an active conformation.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 303-346 from the human protein was used as the immunogen for the STRADA antibody.
Limitation:
This STRADA antibody is available for research use only.
Localization:
Nuclear, cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse
Uniprot #:
Q7RTN6