Synaptophysin Antibody

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NSJ Bioreagents
Product Code: NSJ-F54316
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54316-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54316-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the Synaptophysin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 6
Immunofluorescent staining of human brain tissue with Synaptophysin antibody (green) and DAPI nuclear stain (blue).
2 / 6
IHC testing of FFPE human brain tissue with Synaptophysin antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
3 / 6
Immunofluorescent staining of mouse brain tissue with Synaptophysin antibody (green) and DAPI nuclear stain (blue).
4 / 6
IHC testing of FFPE mouse hippocampus tissue with Synaptophysin antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
5 / 6
Western blot testing of mouse 1) brain and 2) cerebellum lysate with Synaptophysin antibody. Predicted molecular weight: 34-38 kDa.
6 / 6
Flow cytometry testing of mouse Neuro-2a cells with Synaptophysin antibody; Blue=isotype control, Green= Synaptophysin antibody.
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Immunofluorescent staining of human brain tissue with Synaptophysin antibody (green) and DAPI nuclear stain (blue).
IHC testing of FFPE human brain tissue with Synaptophysin antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of mouse brain tissue with Synaptophysin antibody (green) and DAPI nuclear stain (blue).
IHC testing of FFPE mouse hippocampus tissue with Synaptophysin antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of mouse 1) brain and 2) cerebellum lysate with Synaptophysin antibody. Predicted molecular weight: 34-38 kDa.
Flow cytometry testing of mouse Neuro-2a cells with Synaptophysin antibody; Blue=isotype control, Green= Synaptophysin antibody.

Documents

Further Information

Application Details :
Western blot: 1:500-1:2000,Immunohistochemistry (FFPE): 1:25,Immunofluorescence: 1:25,Flow cytometry: 1:25 (1x10e6 cells)
Application Note:
The stated application concentrations are suggested starting points. Titration of the Synaptophysin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Synaptophysin (p38) is an integral membrane protein of small synaptic vesicles in brain and endocrine cells.[supplied by OMIM].
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 225-253 from the human protein was used as the immunogen for the Synaptophysin Antibody.
Limitation:
This Synaptophysin antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse
Uniprot #:
P08247