CD82 Antibody

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NSJ Bioreagents
Product Code: NSJ-F54421
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54421-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54421-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the CD82 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Western blot testing of human Jurkat cell lysate with CD82 antibody. Expected molecular weight: 30-60 kDa depending on glycosylation level.
2 / 5
Western blot testing of human 1) Jurkat and 2) Raji cell lysate with CD82 antibody. Expected molecular weight: 30-60 kDa depending on glycosylation level.
3 / 5
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with CD82 antibody.
4 / 5
IHC testing of FFPE human lymph tissue with CD82 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
5 / 5
Flow cytometry testing of human HepG2 cells with CD82 antibody; Blue=isotype control, Green= CD82 antibody.
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Western blot testing of human Jurkat cell lysate with CD82 antibody. Expected molecular weight: 30-60 kDa depending on glycosylation level.
Western blot testing of human 1) Jurkat and 2) Raji cell lysate with CD82 antibody. Expected molecular weight: 30-60 kDa depending on glycosylation level.
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with CD82 antibody.
IHC testing of FFPE human lymph tissue with CD82 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Flow cytometry testing of human HepG2 cells with CD82 antibody; Blue=isotype control, Green= CD82 antibody.

Documents

Further Information

Application Details :
Immunohistochemistry (FFPE): 1:25,Flow cytometry: 1:25 (1x10e6 cells),Western blot: 1:500-1:2000
Application Note:
The stated application concentrations are suggested starting points. Titration of the CD82 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
The ST6 metastasis suppressor gene product is a membrane glycoprotein that is a member of the transmembrane 4 superfamily. Expression has been shown to be downregulated in tumor progression of human cancers and can be activated by p53 through a consensus binding sequence in the promoter. Its expression and that of p53 are strongly correlated, and the loss of expression of these two proteins is associated with poor survival for prostate cancer patients.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 239-267 from the human protein was used as the immunogen for the CD82 antibody.
Limitation:
This CD82 antibody is available for research use only.
Purity:
SAS precipitation
Species Reactivity :
Human, Mouse
Uniprot #:
P27701