EFL1 Antibody / EFTUD1

NSJ Bioreagents
Product Code: NSJ-F54540
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54540-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54540-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the EFL1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
IHC testing of FFPE human skeletal muscle tissue with EFL1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 4
Western blot testing of human HeLa cell lysate with EFL1 antibody. Predicted molecular weight ~125 kDa.
3 / 4
Western blot testing of mouse spleen tissue lysate with EFL1 antibody. Predicted molecular weight ~125 kDa.
4 / 4
Flow cytometry testing of human HeLa cells with EFL1 antibody; Blue=isotype control, Green= EFL1 antibody.

IHC testing of FFPE human skeletal muscle tissue with EFL1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of human HeLa cell lysate with EFL1 antibody. Predicted molecular weight ~125 kDa.
Western blot testing of mouse spleen tissue lysate with EFL1 antibody. Predicted molecular weight ~125 kDa.
Flow cytometry testing of human HeLa cells with EFL1 antibody; Blue=isotype control, Green= EFL1 antibody.

Further Information

Application Details :
Immunohistochemistry (FFPE): 1:25, Flow cytometry: 1:25 (1x10e6 cells), Western blot: 1:500-1:2000
Application Note:
The stated application concentrations are suggested starting points. Titration of the EFL1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Involved in the biogenesis of the 60S ribosomal subunit and translational activation of ribosomes. Together with SBDS, triggers the GTP-dependent release of EIF6 from 60S pre-ribosomes in the cytoplasm, thereby activating ribosomes for translation competence by allowing 80S ribosome assembly and facilitating EIF6 recycling to the nucleus, where it is required for 60S rRNA processing and nuclear export. Has low intrinsic GTPase activity. GTPase activity is increased by contact with 60S ribosome subunits. [UniProt]
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 1062-1090 from the human protein was used as the immunogen for the EFL1 antibody.
Limitation:
This EFL1 antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse
Uniprot #:
Q7Z2Z2