FGFR4 Antibody

Immunohistochemistry (FFPE): 1:25,Flow cytometry: 1:25 (1x10e6 cells),Western blot: 1:500-1:2000

The stated application concentrations are suggested starting points. Titration of the FGFR4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

FGFR4 is a member of the fibroblast growth factor receptor family, where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein would consist of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. The genomic organization of the gene, compared to members 1-3, encompasses 18 exons rather than 19 or 20. Although alternative splicing has been observed, there is no evidence that the C-terminal half of the IgIII domain of this protein varies between three alternate forms, as indicated for members 1-3. This particular family member preferentially binds acidic fibroblast growth factor and, although its specific function is unknown, it is overexpressed in gynecological tumor samples, suggesting a role in breast and ovarian tumorigenesis.

Purified

In 1X PBS, pH 7.4, with 0.09% sodium azide

A portion of amino acids 24-55 from the human protein was used as the immunogen for the FGFR4 antibody.

This FGFR4 antibody is available for research use only.

Cytoplasmic

SAS precipitation

Human, Mouse

P22455