Hemoglobin subunit gamma-2 Antibody / HBG2

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NSJ Bioreagents
Product Code: NSJ-F54743
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54743-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54743-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the HBG2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
IHC testing of FFPE human liver tissue with HBG2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 4
IHC testing of FFPE mouse liver tissue with HBG2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
3 / 4
Western blot testing of human 1) K562 and 2) placenta lysate with HBG2 antibody. Predicted molecular weight ~16 kDa.
4 / 4
Flow cytometry testing of human U-87 MG cells with HBG2 antibody; Blue=isotype control, Green= HBG2 antibody.
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IHC testing of FFPE human liver tissue with HBG2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE mouse liver tissue with HBG2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of human 1) K562 and 2) placenta lysate with HBG2 antibody. Predicted molecular weight ~16 kDa.
Flow cytometry testing of human U-87 MG cells with HBG2 antibody; Blue=isotype control, Green= HBG2 antibody.

Documents

Further Information

Application Details :
Immunohistochemistry (FFPE): 1:25, Flow cytometry: 1:25 (1x10e6 cells), Western blot: 1:500-1:1000
Application Note:
The stated application concentrations are suggested starting points. Titration of the HBG2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Gamma chains make up the fetal hemoglobin F, in combination with alpha chains. [UniProt]
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 101-133 from the human protein was used as the immunogen for the HBG2 antibody.
Limitation:
This HBG2 antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse
Uniprot #:
P69892