OASIS Antibody / CREB3L1

NSJ Bioreagents
Product Code: NSJ-F54862
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54862-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54862-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the OASIS antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
Western blot testing of human HepG2 cell lysate with OASIS antibody. Predicted molecular weight ~57 kDa. The ~47 kDa isoform may also be visualized.
2 / 4
Western blot testing of mouse stomach tissue lysate with OASIS antibody. Predicted molecular weight ~57 kDa. The ~47 kDa isoform may also be visualized.
3 / 4
IHC testing of FFPE human prostate carcinoma tissue with OASIS antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
4 / 4
Flow cytometry testing of human HeLa cells with OASIS antibody; Blue=isotype control, Green= OASIS antibody.

Western blot testing of human HepG2 cell lysate with OASIS antibody. Predicted molecular weight ~57 kDa. The ~47 kDa isoform may also be visualized.
Western blot testing of mouse stomach tissue lysate with OASIS antibody. Predicted molecular weight ~57 kDa. The ~47 kDa isoform may also be visualized.
IHC testing of FFPE human prostate carcinoma tissue with OASIS antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Flow cytometry testing of human HeLa cells with OASIS antibody; Blue=isotype control, Green= OASIS antibody.

Further Information

Application Details :
Immunohistochemistry (FFPE): 1:50-1:100, Flow cytometry: 1:10-1:50 (1x10e6 cells), Western blot: 1:2000-1:8000
Application Note:
The stated application concentrations are suggested starting points. Titration of the OASIS antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
CREB3L1 is a transcription factor that acts during endoplasmic reticulum stress by activating unfolded protein response target genes. It is specifically involved in ER-stress response in astrocytes in the central nervous system (By similartity). It may play a role in gliosis. In vitro, it binds to box-B element, cAMP response element (CRE) and CRE-like sequences, and activates transcription through box-B element but not through CRE.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 481-509 from the human protein was used as the immunogen for the OASIS antibody.
Limitation:
This OASIS antibody is available for research use only.
Localization:
Cytoplasmic, nuclear
Purity:
Purified
Species Reactivity :
Human, Mouse
Uniprot #:
Q96BA8