COL8A2 Antibody

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NSJ Bioreagents
Product Code: NSJ-F54619
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54619-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54619-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the COL8A2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
Western blot testing of 1) human SH-SY5Y, 2) human Jurkat, 3) mouse Neuro-2a and 4) mouse C2C12 cell lysate with COL8A2 antibody. Predicted molecular weight ~67 kDa.
2 / 4
IHC testing of FFPE human testis tissue with COL8A2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
3 / 4
IHC testing of FFPE human kidney tissue with COL8A2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
4 / 4
Immunofluorescent staining of human HepG2 cells with COL8A2 antibody (green) and DAPI nuclear stain (blue).
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Western blot testing of 1) human SH-SY5Y, 2) human Jurkat, 3) mouse Neuro-2a and 4) mouse C2C12 cell lysate with COL8A2 antibody. Predicted molecular weight ~67 kDa.
IHC testing of FFPE human testis tissue with COL8A2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE human kidney tissue with COL8A2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of human HepG2 cells with COL8A2 antibody (green) and DAPI nuclear stain (blue).

Documents

Further Information

Application Details :
Immunohistochemistry (FFPE): 1:25, Immunofluorescence: 1:25, Western blot: 1:500-1:2000
Application Note:
The stated application concentrations are suggested starting points. Titration of the COL8A2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This gene encodes the alpha 2 chain of type VIII collagen. The protein is a major component of the basement membrane of the corneal endothelium and forms homo- or heterotrimers with alpha 1 (VIII) type collagens. Defects in this gene are associated with Fuchs endothelial corneal dystrophy and posterior polymorphous corneal dystrophy type 2.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 571-599 from the human protein was used as the immunogen for the COL8A2 antibody.
Limitation:
This COL8A2 antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse
Uniprot #:
P25067