Cytochrome C1 Antibody / CYC1

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NSJ Bioreagents
Product Code: NSJ-F54386
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54386-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54386-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the Cytochrome C1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 5
Western blot testing of human 1) A431, 2) HeLa, 3) HepG2, 4) MCF7, 5) U-251 MG and 6) mouse brain lysate with Cytochrome C1 antibody. Predicted molecular weight ~35 kDa.
2 / 5
IHC testing of FFPE human stomach tissue with Cytochrome C1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
3 / 5
IHC testing of FFPE mouse stomach tissue with Cytochrome C1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
4 / 5
IHC testing of FFPE rat stomach tissue with Cytochrome C1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
5 / 5
Immunofluorescent staining of fixed and permeabilized human HeLa cells with Cytochrome C1 antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
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Western blot testing of human 1) A431, 2) HeLa, 3) HepG2, 4) MCF7, 5) U-251 MG and 6) mouse brain lysate with Cytochrome C1 antibody. Predicted molecular weight ~35 kDa.
IHC testing of FFPE human stomach tissue with Cytochrome C1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE mouse stomach tissue with Cytochrome C1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE rat stomach tissue with Cytochrome C1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of fixed and permeabilized human HeLa cells with Cytochrome C1 antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).

Documents

Further Information

Application Details :
Immunohistochemistry (FFPE): 1:25,Immunofluorescence: 1:25,Western blot: 1:500-1:2000
Application Note:
The stated application concentrations are suggested starting points. Titration of the Cytochrome C1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This is the heme-containing component of the cytochrome b-c1 complex, which accepts electrons from Rieske protein and transfers electrons to cytochrome c in the mitochondrial respiratory chain.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 142-176 from the human protein was used as the immunogen for the Cytochrome C1 antibody.
Limitation:
This Cytochrome C1 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse
Uniprot #:
P08574