Exportin 2 antibody / CSE1L

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NSJ Bioreagents
Product Code: NSJ-F54382
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54382-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54382-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the Exportin 2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
Western blot testing of human 1) HeLa, 2) Jurkat, 3) Ramos and 4) mouse NIH 3T3 cell lysate with Exportin 2 antibody. Predicted molecular weight ~110 kDa.
2 / 4
IHC testing of FFPE human stomach tissue with Exportin 2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
3 / 4
IHC testing of FFPE human tonsil tissue with Exportin 2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
4 / 4
Immunofluorescent staining of human HeLa cells with Exportin 2 antibody (green) and anti-Actin (red).
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Western blot testing of human 1) HeLa, 2) Jurkat, 3) Ramos and 4) mouse NIH 3T3 cell lysate with Exportin 2 antibody. Predicted molecular weight ~110 kDa.
IHC testing of FFPE human stomach tissue with Exportin 2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE human tonsil tissue with Exportin 2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of human HeLa cells with Exportin 2 antibody (green) and anti-Actin (red).

Documents

Further Information

Application Details :
Immunohistochemistry (FFPE): 1:25,Immunofluorescence: 1:25,Western blot: 1:500-1:2000
Application Note:
The stated application concentrations are suggested starting points. Titration of the Exportin 2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Proteins that carry a nuclear localization signal (NLS) are transported into the nucleus by the importin-alpha/beta heterodimer. Importin-alpha binds the NLS, while importin-beta mediates translocation through the nuclear pore complex. After translocation, RanGTP binds importin-beta and displaces importin-alpha. Importin-alpha must then be returned to the cytoplasm, leaving the NLS protein behind. CSE1L binds strongly to NLS-free importin-alpha, and this binding is released in the cytoplasm by the combined action of RANBP1 and RANGAP1. In addition, CSE1L may play a role both in apoptosis and in cell proliferation.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 55-84 from the human protein was used as the immunogen for the Exportin 2 antibody.
Limitation:
This Exportin 2 antibody is available for research use only.
Localization:
Nuclear, cytoplasmic
Purity:
SAS precipitation
Species Reactivity :
Human, Mouse
Uniprot #:
P55060