Splicing factor 3B subunit 1 Antibody / SF3B1

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NSJ Bioreagents
Product Code: NSJ-F54673
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54673-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54673-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the Splicing factor 3B subunit 1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Western blot testing of 1) human PANC-1, 2) human HeLa, 3) human Jurkat, 4) mouse NIH 3T3, 5) human H-4-II-E, 6) human A431 and 7) human HepG2 cell lysate with Splicing factor 3B subunit 1 antibody. Predicted molecular weight ~146 kDa.
2 / 4
Western blot testing of human CCRF-CEM cell lysate with Splicing factor 3B subunit 1 antibody. Predicted molecular weight ~146 kDa.
3 / 4
IHC testing of FFPE human esophagus carcinoma tissue with Splicing factor 3B subunit 1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
4 / 4
IHC testing of FFPE human tonsil tissue with Splicing factor 3B subunit 1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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Western blot testing of 1) human PANC-1, 2) human HeLa, 3) human Jurkat, 4) mouse NIH 3T3, 5) human H-4-II-E, 6) human A431 and 7) human HepG2 cell lysate with Splicing factor 3B subunit 1 antibody. Predicted molecular weight ~146 kDa.
Western blot testing of human CCRF-CEM cell lysate with Splicing factor 3B subunit 1 antibody. Predicted molecular weight ~146 kDa.
IHC testing of FFPE human esophagus carcinoma tissue with Splicing factor 3B subunit 1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE human tonsil tissue with Splicing factor 3B subunit 1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Documents

Further Information

Application Details :
Immunohistochemistry (FFPE): 1:25, Western blot: 1:500-1:2000
Application Note:
The stated application concentrations are suggested starting points. Titration of the Splicing factor 3B subunit 1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This gene encodes subunit 1 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. The carboxy-terminal two-thirds of subunit 1 have 22 non-identical, tandem HEAT repeats that form rod-like, helical structures. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq].
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 374-402 from the human protein was used as the immunogen for the Splicing factor 3B subunit 1 antibody.
Limitation:
This Splicing factor 3B subunit 1 antibody is available for research use only.
Localization:
Nuclear
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse
Uniprot #:
O75533