Albumin Antibody / ALB

NSJ Bioreagents
Product Code: NSJ-F54330
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54330-0.05ML0.05 ml£260.00
Quantity:
NSJ-F54330-0.2ML0.2 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the Albumin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
Western blot testing of 1) human HepG2, 2) human HeLa and 3) mouse liver lysate with Albumin antibody. Predicted molecular weight ~66 kDa.
2 / 4
IHC testing of FFPE human kidney tissue with Albumin antibody. HIER: steam section in pH9 EDTA for 20 min and allow to cool prior to staining.
3 / 4
IHC testing of FFPE human liver tissue with Albumin antibody. HIER: steam section in pH9 EDTA for 20 min and allow to cool prior to staining.
4 / 4
Flow cytometry testing of human HepG2 cells with Albumin antibody; Blue=isotype control, Green= Albumin antibody.

Western blot testing of 1) human HepG2, 2) human HeLa and 3) mouse liver lysate with Albumin antibody. Predicted molecular weight ~66 kDa.
IHC testing of FFPE human kidney tissue with Albumin antibody. HIER: steam section in pH9 EDTA for 20 min and allow to cool prior to staining.
IHC testing of FFPE human liver tissue with Albumin antibody. HIER: steam section in pH9 EDTA for 20 min and allow to cool prior to staining.
Flow cytometry testing of human HepG2 cells with Albumin antibody; Blue=isotype control, Green= Albumin antibody.

Further Information

Application Details :
Immunohistochemistry (FFPE): 1:25,Western blot: 1:500-1:2000,Flow cytometry: 1:25 (1x10e6 cells)
Application Note:
The stated application concentrations are suggested starting points. Titration of the Albumin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Albumin is a soluble, monomeric protein which comprises about one-half of the blood serum protein. Albumin functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Albumin is a globular unglycosylated serum protein of molecular weight 65,000. Albumin is synthesized in the liver as preproalbumin which has an N-terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 540-569 from the human protein was used as the immunogen for the Albumin antibody.
Limitation:
This Albumin antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse
Uniprot #:
P02768