Angiotensin II Type 1 Receptor Antibody / AGTR1 / AT1R

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NSJ Bioreagents
Product Code: NSJ-F54336
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54336-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54336-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the Angiotensin II Type 1 Receptor antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 5
IHC testing of FFPE human brain tissue with Angiotensin II Type 1 Receptor antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 5
Immunofluorescent staining of fixed and permeabilized human HepG2 cells with Cytokeratin 18 antibody (green) and DAPI nuclear stain (blue).
3 / 5
Western blot testing of mouse NIH 3T3 cell lysate with Angiotensin II Type 1 Receptor antibody. Observed molecular weight: 41-50 kDa depending on glycosylation level.
4 / 5
Western blot testing of human HepG2 cell lysate with Angiotensin II Type 1 Receptor antibody. Observed molecular weight: 41-50 kDa depending on glycosylation level.
5 / 5
Flow cytometry testing of fixed and permeabilized human HepG2 cells with Angiotensin II Type 1 Receptor antibody; Blue=isotype control, Green= Angiotensin II Type 1 Receptor antibody.
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IHC testing of FFPE human brain tissue with Angiotensin II Type 1 Receptor antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of fixed and permeabilized human HepG2 cells with Cytokeratin 18 antibody (green) and DAPI nuclear stain (blue).
Western blot testing of mouse NIH 3T3 cell lysate with Angiotensin II Type 1 Receptor antibody. Observed molecular weight: 41-50 kDa depending on glycosylation level.
Western blot testing of human HepG2 cell lysate with Angiotensin II Type 1 Receptor antibody. Observed molecular weight: 41-50 kDa depending on glycosylation level.
Flow cytometry testing of fixed and permeabilized human HepG2 cells with Angiotensin II Type 1 Receptor antibody; Blue=isotype control, Green= Angiotensin II Type 1 Receptor antibody.

Documents

Further Information

Application Details :
Immunohistochemistry (FFPE): 1:25,Western blot: 1:500-1:2000,Flow cytometry: 1:25 (1x10e6 cells),Immunofluorescence: 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the Angiotensin II Type 1 Receptor antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Angiotensin II is a potent vasopressor hormone and a primary regulator of aldosterone secretion. It is an important effector controlling blood pressure and volume in the cardiovascular system. It acts through at least two types of receptors. This gene encodes the type 1 receptor which is thought to mediate the major cardiovascular effects of angiotensin II. This gene may play a role in the generation of reperfusion arrhythmias following restoration of blood flow to ischemic or infarcted myocardium. It was previously thought that a related gene, denoted as AGTR1B, existed; however, it is now believed that there is only one type 1 receptor gene in humans. At least five transcript variants have been described for this gene. Additional variants have been described but their full-length nature has not been determined. The entire coding sequence is contained in the terminal exon and is present in all transcript variants. [provided by RefSeq].
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 211-240 from the human protein was used as the immunogen for the Angiotensin II Type 1 Receptor antibody.
Limitation:
This Angiotensin II Type 1 Receptor antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse
Uniprot #:
P30556