Haptoglobin Antibody

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NSJ Bioreagents
Product Code: NSJ-F54341
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54341-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54341-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the Haptoglobin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 5
IHC testing of FFPE human hepatocarcinoma tissue with Haptoglobin antibody. HIER: steam section in pH9 EDTA for 20 min and allow to cool prior to staining.
2 / 5
IHC testing of FFPE human colon tissue with Haptoglobin antibody. HIER: steam section in pH9 EDTA for 20 min and allow to cool prior to staining.
3 / 5
Immunofluorescent staining of fixed and permeabilized human MDA-MB-435 cells with Haptoglobin antibody (green) and DAPI nuclear stain (blue).
4 / 5
Western blot testing of 1) human liver, 2) human plasma and 3) mouse plasma lysate with Haptoglobin antibody. Predicted molecular weight ~45 kDa.
5 / 5
Flow cytometry testing of human MDA-MB-435 cells with Haptoglobin antibody; Blue=isotype control, Green= Haptoglobin antibody.
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IHC testing of FFPE human hepatocarcinoma tissue with Haptoglobin antibody. HIER: steam section in pH9 EDTA for 20 min and allow to cool prior to staining.
IHC testing of FFPE human colon tissue with Haptoglobin antibody. HIER: steam section in pH9 EDTA for 20 min and allow to cool prior to staining.
Immunofluorescent staining of fixed and permeabilized human MDA-MB-435 cells with Haptoglobin antibody (green) and DAPI nuclear stain (blue).
Western blot testing of 1) human liver, 2) human plasma and 3) mouse plasma lysate with Haptoglobin antibody. Predicted molecular weight ~45 kDa.
Flow cytometry testing of human MDA-MB-435 cells with Haptoglobin antibody; Blue=isotype control, Green= Haptoglobin antibody.

Documents

Further Information

Application Details :
Immunohistochemistry (FFPE): 1:25,Western blot: 1:500-1:2000,Immunofluorescence: 1:25,Flow cytometry: 1:25 (1x10e6 cells)
Application Note:
The stated application concentrations are suggested starting points. Titration of the Haptoglobin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This gene encodes a preproprotein, which is processed to yield both alpha and beta chains, which subsequently combine as a tetramer to produce haptoglobin. Haptoglobin functions to bind free plasma hemoglobin, which allows degradative enzymes to gain access to the hemoglobin, while at the same time preventing loss of iron through the kidneys and protecting the kidneys from damage by hemoglobin. Mutations in this gene and/or its regulatory regions cause ahaptoglobinemia or hypohaptoglobinemia. This gene has also been linked to diabetic nephropathy, the incidence of coronary artery disease in type 1 diabetes, Crohn's disease, inflammatory disease behavior, primary sclerosing cholangitis, susceptibility to idiopathic Parkinson's disease, and a reduced incidence of Plasmodium falciparum malaria. A similar duplicated gene is located next to this gene on chromosome 16. Multiple transcript variants encoding different isoforms have been found for this gene.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 296-322 from the human protein was used as the immunogen for the Haptoglobin antibody.
Limitation:
This Haptoglobin antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse
Uniprot #:
P00738