Phospholipid-transporting ATPase IG Antibody / ATP11C

NSJ Bioreagents
Product Code: NSJ-F54825
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54825-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54825-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Hamster
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the Phospholipid-transporting ATPase IG antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
IHC testing of FFPE human prostate carcinoma tissue with Phospholipid-transporting ATPase IG antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 4
Western blot testing of hamster CHO lysate with Phospholipid-transporting ATPase IG antibody. Predicted molecular weight ~129 kDa.
3 / 4
Western blot testing of mouse lung tissue lysate with Phospholipid-transporting ATPase IG antibody. Predicted molecular weight ~129 kDa.
4 / 4
Flow cytometry testing of human HEK293 cells with Phospholipid-transporting ATPase IG antibody; Blue=isotype control, Green= Phospholipid-transporting ATPase IG antibody.

IHC testing of FFPE human prostate carcinoma tissue with Phospholipid-transporting ATPase IG antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of hamster CHO lysate with Phospholipid-transporting ATPase IG antibody. Predicted molecular weight ~129 kDa.
Western blot testing of mouse lung tissue lysate with Phospholipid-transporting ATPase IG antibody. Predicted molecular weight ~129 kDa.
Flow cytometry testing of human HEK293 cells with Phospholipid-transporting ATPase IG antibody; Blue=isotype control, Green= Phospholipid-transporting ATPase IG antibody.

Further Information

Application Details :
Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunohistochemistry (FFPE): 1:50-1:100, Western blot: 1:500-1:1000
Application Note:
The stated application concentrations are suggested starting points. Titration of the Phospholipid-transporting ATPase IG antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
TCatalytic component of a P4-ATPase flippase complex which catalyzes the hydrolysis of ATP coupled to the transport of aminophospholipids, phosphatidylserines (PS) and phosphatidylethanolamines (PE), from the outer to the inner leaflet of the plasma membrane. [UniProt]
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 589-616 from the human protein was used as the immunogen for the Phospholipid-transporting ATPase IG antibody.
Limitation:
This Phospholipid-transporting ATPase IG antibody is available for research use only.
Localization:
Cytoplasmic, plasma membrane
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse, Hamster
Uniprot #:
Q8NB49