MMP14 Antibody

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NSJ Bioreagents
Product Code: NSJ-F54844
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54844-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54844-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the MMP14 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 3
IHC testing of FFPE human lung adenocarcinoma tissue with MMP14 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 3
Western blot testing of 1) human A2058, 2) human HT-1080, 3) human MCF-7, 4) human NCI-H292, 5) mouse kidney and 6) rat kidney tissue lysate with MMP14 antibody. Predicted molecular weight ~66 kDa (pro form), ~54 kDa (active form), ~44 kDa (inactive form).
3 / 3
Flow cytometry testing of human MCF7 cells with MMP14 antibody; Blue=isotype control, Green= MMP14 antibody.
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IHC testing of FFPE human lung adenocarcinoma tissue with MMP14 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of 1) human A2058, 2) human HT-1080, 3) human MCF-7, 4) human NCI-H292, 5) mouse kidney and 6) rat kidney tissue lysate with MMP14 antibody. Predicted molecular weight ~66 kDa (pro form), ~54 kDa (active form), ~44 kDa (inactive form).
Flow cytometry testing of human MCF7 cells with MMP14 antibody; Blue=isotype control, Green= MMP14 antibody.

Documents

Further Information

Application Details :
Western blot: 1:500-1:1000, Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunohistochemistry (FFPE): 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the MMP14 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMPs are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. MMP14 seems to specifically activate progelatinase A, and may thus trigger invasion by tumor cells by activating progelatinase A on the tumor cell surface. Expression is significant in stromal cells of colon, breast, and head and neck.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 145-174 from the human protein was used as the immunogen for the MMP14 antibody.
Limitation:
This MMP14 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P50281