NSJ Bioreagents

Vinculin Antibody

Product Code:
 
NSJ-F54510
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Aliquot the Vinculin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 4
Flow cytometry testing of fixed and permeabilized mouse NIH 3T3 cells with Vinculin antibody; Blue=isotype control, Green= Vinculin antibody.
2 / 4
Flow cytometry testing of fixed and permeabilized mouse C2C12 cells with Vinculin antibody; Blue=isotype control, Green= Vinculin antibody.
3 / 4
Western blot testing of 1) human skeletal muscle, 2) human A431, 3) human HeLa, 4) mouse kidney, 5) rat PC-12 and 6) human HepG2 cell lysate with Vinculin antibody. Predicted molecular weight ~124 kDa.
4 / 4
IHC testing of FFPE mouse skeletal muscle tissue with Vinculin antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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Flow cytometry testing of fixed and permeabilized mouse NIH 3T3 cells with Vinculin antibody; Blue=isotype control, Green= Vinculin antibody.
Flow cytometry testing of fixed and permeabilized mouse C2C12 cells with Vinculin antibody; Blue=isotype control, Green= Vinculin antibody.
Western blot testing of 1) human skeletal muscle, 2) human A431, 3) human HeLa, 4) mouse kidney, 5) rat PC-12 and 6) human HepG2 cell lysate with Vinculin antibody. Predicted molecular weight ~124 kDa.
IHC testing of FFPE mouse skeletal muscle tissue with Vinculin antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

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CodeSizePrice
NSJ-F54510-0.05ML0.05 ml£260.00
Quantity:
NSJ-F54510-0.2ML0.2 ml£502.00
Quantity:
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Further Information

Application Details :
Western blot: 1:500-1:2000, Flow cytometry: 1:25 (1x10e6 cells), Immunohistochemistry (FFPE): 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the Vinculin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell- surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion (By similarity).
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
Recombinant human protein was used as the immunogen for the Vinculin antibody.
Limitation:
This Vinculin antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q64727