HSC70 Antibody / HSPA8

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NSJ Bioreagents
Product Code: NSJ-F54784
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54784-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54784-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the HSC70 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
Flow cytometry testing of human HeLa cells with HSC70 antibody; Blue=isotype control, Green= HSC70 antibody.
2 / 4
Immunofluorescent staining of human HeLa cells with HSC70 antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
3 / 4
Western blot testing of 1) rat H-4-II-E and 2) mouse NIH 3T3 cell lysate with HSPA8 antibody. Expected molecular weight: 70-73 kDa.
4 / 4
IHC testing of FFPE human stomach tissue with HSC70 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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Flow cytometry testing of human HeLa cells with HSC70 antibody; Blue=isotype control, Green= HSC70 antibody.
Immunofluorescent staining of human HeLa cells with HSC70 antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
Western blot testing of 1) rat H-4-II-E and 2) mouse NIH 3T3 cell lysate with HSPA8 antibody. Expected molecular weight: 70-73 kDa.
IHC testing of FFPE human stomach tissue with HSC70 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Documents

Further Information

Application Details :
Western blot: 1:500-1:1000, Flow cytometry: 1:25 (1x10e6 cells), Immunohistochemistry (FFPE): 1:25, Immunofluorescence: 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the HSC70 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
HSPA8 / HSC70 belongs to the heat shock protein 70 family which contains both heat-inducible and constitutively expressed members. The latter are called heat-shock cognate proteins. HSPA8 is a heat-shock cognate protein. This protein binds to nascent polypeptides to facilitate correct folding. The protein also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 539-569 from the human protein was used as the immunogen for the HSC70 antibody.
Limitation:
This HSC70 antibody is available for research use only.
Localization:
Cytoplasmic, nuclear
Purity:
Purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P11142