NSJ Bioreagents

OAT Antibody

Product Code:
 
NSJ-F54467
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Aliquot the OAT antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 5
Western blot testing of 1) human HEK293, 2) human HEK293T, 3) mouse liver and 4) rat liver lysate with OAT antibody. Predicted molecular weight ~49 kDa.
2 / 5
Western blot testing of human 1) HEK293 and 2) liver lysate with OAT antibody. Predicted molecular weight ~49 kDa.
3 / 5
Immunofluorescent staining of fixed and permeabilized human A549 cells with OAT antibody (green) and anti-Actin (red).
4 / 5
IHC testing of FFPE human brain tissue with OAT antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
5 / 5
Flow cytometry testing of human HEK293 cells with OAT antibody; Blue=isotype control, Green= OAT antibody.
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Western blot testing of 1) human HEK293, 2) human HEK293T, 3) mouse liver and 4) rat liver lysate with OAT antibody. Predicted molecular weight ~49 kDa.
Western blot testing of human 1) HEK293 and 2) liver lysate with OAT antibody. Predicted molecular weight ~49 kDa.
Immunofluorescent staining of fixed and permeabilized human A549 cells with OAT antibody (green) and anti-Actin (red).
IHC testing of FFPE human brain tissue with OAT antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Flow cytometry testing of human HEK293 cells with OAT antibody; Blue=isotype control, Green= OAT antibody.

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CodeSizePrice
NSJ-F54467-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54467-0.4ML0.4 ml£502.00
Quantity:
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Further Information

Application Details :
Western blot: 1:500-1:2000,Immunofluorescence: 1:25,Flow cytometry: 1:25 (1x10e6 cells),Immunohistochemistry (FFPE): 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the OAT antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
OAT is the mitochondrial enzyme ornithine aminotransferase, which is a key enzyme in the pathway that converts arginine and ornithine into the major excitatory and inhibitory neurotransmitters glutamate and GABA.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 27-55 from the human protein was used as the immunogen for the OAT antibody.
Limitation:
This OAT antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P04181