NSJ Bioreagents

PCK1 Antibody / PEPCK1 / PEPC

Product Code:
 
NSJ-F54453
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Aliquot the PEPCK1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 3
Western blot testing of 1) human liver, 2) human kidney, 3) human NCI-H460, 4) mouse liver, 5) mouse kidney and 6) rat kidney lysate with PEPCK1 antibody. Predicted molecular weight: ~69 kDa.
2 / 3
IHC testing of FFPE human kidney tissue with PEPCK1 antibody. HIER: steam section in pH9 EDTA buffer for 20 min and allow to cool prior to staining.
3 / 3
Immunofluorescent staining of human HepG2 cells with PEPCK1 antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
❮❯

Western blot testing of 1) human liver, 2) human kidney, 3) human NCI-H460, 4) mouse liver, 5) mouse kidney and 6) rat kidney lysate with PEPCK1 antibody. Predicted molecular weight: ~69 kDa.
IHC testing of FFPE human kidney tissue with PEPCK1 antibody. HIER: steam section in pH9 EDTA buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of human HepG2 cells with PEPCK1 antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).

No additional charges, what you see is what you pay! *

CodeSizePrice
NSJ-F54453-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54453-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT
How to order with us   Contact us
Stay in control of your spending. These prices have no additional charges, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available! Contact us to find what you can save.
This product comes from: United States.
Typical lead time: 10-14 working days.
Contact us for more accurate information.
  • Further Information
  • Documents
  • Show All

Further Information

Application Details :
Western blot: 1:500-1:2000,Immunofluorescence: 1:25,Immunohistochemistry (FFPE): 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the PEPCK1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This gene is a main control point for the regulation of gluconeogenesis. The cytosolic enzyme encoded by this gene, along with GTP, catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. The expression of this gene can be regulated by insulin, glucocorticoids, glucagon, cAMP, and diet. A mitochondrial isozyme of the encoded protein also has been characterized.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 592-622 from the human protein was used as the immunogen for the PEPCK1 antibody.
Limitation:
This PEPCK1 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P35558