GCK Antibody / Glucokinase (N-Terminal Region)

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NSJ Bioreagents
Product Code: NSJ-F54202
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54202-0.05ML0.05 ml£260.00
Quantity:
NSJ-F54202-0.2ML0.2 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the GCK antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

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Immunofluorescence testing of human HepG2 cells with GCK antibody (green) and DAPI nuclear counterstain (blue).
2 / 3
Western blot testing of 1) human HepG2 and 2) rat liver lysate with GCK antibody. Predicted molecular weight ~52 kDa.
3 / 3
IHC testing of FFPE human breast carcinoma tissue with GCK antibody.
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Immunofluorescence testing of human HepG2 cells with GCK antibody (green) and DAPI nuclear counterstain (blue).
Western blot testing of 1) human HepG2 and 2) rat liver lysate with GCK antibody. Predicted molecular weight ~52 kDa.
IHC testing of FFPE human breast carcinoma tissue with GCK antibody.

Documents

Further Information

Application Details :
Western Blot: 1:500-1:1000,Immunohistochemistry (FFPE): 1:50-1:100,Immunofluorescence: 1:10-1:50
Application Note:
Optimal dilution of the GCK antibody should be determined by the researcher.
Description:
Hexokinases phosphorylate glucose to produce glucose-6-phosphate, thus committing glucose to the glycolytic pathway. Alternative splicing of the gene for GCK results in three tissue-specific forms of glucokinase, one found in pancreatic islet beta cells and two found in liver. The protein localizes to the outer membrane of mitochondria. In contrast to other forms of hexokinase, this enzyme is not inhibited by its product glucose-6-phosphate but remains active while glucose is abundant. Mutations in the gene have been associated with non-insulin dependent diabetes mellitus (NIDDM), also called maturity onset diabetes of the young, type 2 (MODY2); mutations have also been associated with persistent hyperinsulinemic hypoglycemia of infancy (PHHI).
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 1-30 from the human protein was used as the immunogen for the GCK antibody.
Limitation:
This GCK antibody is available for research use only.
Localization:
Cytoplasmic, nuclear
Purity:
Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis in 1X PBS
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P35557