CBS Antibody / Cystathionine Beta Synthase

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NSJ Bioreagents
Product Code: NSJ-F54306
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54306-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54306-0.4ML0.4 ml£480.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the CBS antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 5
IHC testing of FFPE human brain tissue with CBS antibody. HIER: steam section in pH9 EDTA for 20 min and allow to cool prior to staining.
2 / 5
IHC testing of FFPE human liver tissue with CBS antibody. HIER: steam section in pH9 EDTA for 20 min and allow to cool prior to staining.
3 / 5
Immunofluorescent staining of fixed and permeabilized human HeLa cells with CBS antibody (green) and DAPI nuclear stain (blue).
4 / 5
Western blot testing of human 1) Raji, 2) HeLa, 3) MCF7, 4) LNCaP, 5) human liver, 6) mouse liver and 7) mouse pancreas lysate with CBS antibody. Predicted molecular weight ~61 kDa.
5 / 5
Flow cytometry testing of fixed and permeabilized human HeLa cells with CBS antibody; Blue=isotype control, Green= CBS antibody.
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IHC testing of FFPE human brain tissue with CBS antibody. HIER: steam section in pH9 EDTA for 20 min and allow to cool prior to staining.
IHC testing of FFPE human liver tissue with CBS antibody. HIER: steam section in pH9 EDTA for 20 min and allow to cool prior to staining.
Immunofluorescent staining of fixed and permeabilized human HeLa cells with CBS antibody (green) and DAPI nuclear stain (blue).
Western blot testing of human 1) Raji, 2) HeLa, 3) MCF7, 4) LNCaP, 5) human liver, 6) mouse liver and 7) mouse pancreas lysate with CBS antibody. Predicted molecular weight ~61 kDa.
Flow cytometry testing of fixed and permeabilized human HeLa cells with CBS antibody; Blue=isotype control, Green= CBS antibody.

Documents

Further Information

Application Details :
Western blot: 1:500-1:2000,Immunohistochemistry (FFPE): 1:25,Immunofluorescence: 1:25,Flow cytometry: 1:25 (1x10e6 cells)
Application Note:
The stated application concentrations are suggested starting points. Titration of the CBS antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
CBS acts as a homotetramer to catalyze the conversion of homocysteine to cystathionine, the first step in the transsulfuration pathway. This protein is allosterically activated by adenosyl-methionine and uses pyridoxal phosphate as a cofactor. Defects in this gene can cause cystathionine beta-synthase deficiency (CBSD), which can lead to homocystinuria.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 301-330 from the human protein were used as the immunogen for the CBS antibody.
Limitation:
This CBS antibody is available for research use only.
Localization:
Nuclear, cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P35520