RPS18 Antibody

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NSJ Bioreagents
Product Code: NSJ-F54304
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54304-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54304-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the RPS18 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 6
IHC testing of FFPE human kidney tissue with RPS18 antibody. HIER: steam section in pH9 EDTA for 20 min and allow to cool prior to staining.
2 / 6
IHC testing of FFPE human kidney tissue with RPS18 antibody.
3 / 6
IHC testing of FFPE human liver tissue with RPS18 antibody. HIER: steam section in pH9 EDTA for 20 min and allow to cool prior to staining.
4 / 6
Immunofluorescent staining of fixed and permeabilized human U-2 OS cells with RPS18 antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
5 / 6
Western blot testing of human 1) U-251 MG, 2) HepG2, 3) ThP-1, 4) Daudi, 5) mouse brain, 6) mouse spleen and 7) rat brain lysate with RPS18 antibody. Predicted molecular weight ~18 kDa.
6 / 6
Flow cytometry testing of fixed and permeabilized human U-2 OS cells with RPS18 antibody; Blue=isotype control, Green= RPS18 antibody.
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IHC testing of FFPE human kidney tissue with RPS18 antibody. HIER: steam section in pH9 EDTA for 20 min and allow to cool prior to staining.
IHC testing of FFPE human kidney tissue with RPS18 antibody.
IHC testing of FFPE human liver tissue with RPS18 antibody. HIER: steam section in pH9 EDTA for 20 min and allow to cool prior to staining.
Immunofluorescent staining of fixed and permeabilized human U-2 OS cells with RPS18 antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
Western blot testing of human 1) U-251 MG, 2) HepG2, 3) ThP-1, 4) Daudi, 5) mouse brain, 6) mouse spleen and 7) rat brain lysate with RPS18 antibody. Predicted molecular weight ~18 kDa.
Flow cytometry testing of fixed and permeabilized human U-2 OS cells with RPS18 antibody; Blue=isotype control, Green= RPS18 antibody.

Documents

Further Information

Application Details :
Western blot: 1:500-1:2000,Immunohistochemistry (FFPE): 1:25,Immunofluorescence: 1:25,Flow cytometry: 1:25 (1x10e6 cells)
Application Note:
The stated application concentrations are suggested starting points. Titration of the RPS18 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This protein is a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S13P family of ribosomal proteins. It is located in the cytoplasm.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 62-91 from the human protein were used as the immunogen for the RPS18 antibody.
Limitation:
This RPS18 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P62269