Bleomycin hydrolase Antibody / BLMH

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NSJ Bioreagents
Product Code: NSJ-F54739
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54739-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54739-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the Bleomycin hydrolase antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Western blot testing of human 1) HL60, 2) CCRF-CEM, 3) SH-SY5Y, 4) MOLT4 and 5) mouse Neuro-2a cell lysate with Bleomycin hydrolase antibody. Predicted molecular weight ~53 kDa.
2 / 3
Western blot testing of 1) human L562, 2) mouse stomach and 3) rat stomach lysate with Bleomycin hydrolase antibody. Predicted molecular weight ~53 kDa.
3 / 3
IHC testing of FFPE human skin tissue with Bleomycin hydrolase antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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Western blot testing of human 1) HL60, 2) CCRF-CEM, 3) SH-SY5Y, 4) MOLT4 and 5) mouse Neuro-2a cell lysate with Bleomycin hydrolase antibody. Predicted molecular weight ~53 kDa.
Western blot testing of 1) human L562, 2) mouse stomach and 3) rat stomach lysate with Bleomycin hydrolase antibody. Predicted molecular weight ~53 kDa.
IHC testing of FFPE human skin tissue with Bleomycin hydrolase antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Documents

Further Information

Application Details :
Immunohistochemistry (FFPE): 1:25, Western blot: 1:500-1:1000
Application Note:
The stated application concentrations are suggested starting points. Titration of the Bleomycin hydrolase antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
The normal physiological role of BLM hydrolase is unknown, but it catalyzes the inactivation of the antitumor drug BLM (a glycopeptide) by hydrolyzing the carboxamide bond of its B-aminoalaninamide moiety thus protecting normal and malignant cells from BLM toxicity (By similarity).
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 212-242 from the human protein was used as the immunogen for the Bleomycin hydrolase antibody.
Limitation:
This Bleomycin hydrolase antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q13867