EHD2 Antibody

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NSJ Bioreagents
Product Code: NSJ-F54752
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54752-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54752-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the EHD2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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IHC testing of FFPE human placental tissue with EHD2 antibody. HIER: steam section in pH9 EDTA for 20 min and allow to cool prior to staining.
2 / 3
IHC testing of FFPE human heart tissue with EHD2 antibody. HIER: steam section in pH9 EDTA for 20 min and allow to cool prior to staining.
3 / 3
Western blot testing of human 1) heart, 2) A549, 3) placenta, 4) HeLa, 5) mouse kidney and 6) rat lung tissue lysate with EHD2 antibody. Predicted molecular weight: ~61 kDa.
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IHC testing of FFPE human placental tissue with EHD2 antibody. HIER: steam section in pH9 EDTA for 20 min and allow to cool prior to staining.
IHC testing of FFPE human heart tissue with EHD2 antibody. HIER: steam section in pH9 EDTA for 20 min and allow to cool prior to staining.
Western blot testing of human 1) heart, 2) A549, 3) placenta, 4) HeLa, 5) mouse kidney and 6) rat lung tissue lysate with EHD2 antibody. Predicted molecular weight: ~61 kDa.

Documents

Further Information

Application Details :
Immunohistochemistry (FFPE): 1:25, Western blot: 1:500-1:2000
Application Note:
The stated application concentrations are suggested starting points. Titration of the EHD2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
EH domain-containing protein 2 plays a role in membrane reorganization in response to nucleotide hydrolysis. Binds to liposomes and deforms them into tubules. Plays a role in membrane trafficking between the plasma membrane and endosomes. Important for the internalization of GLUT4. Required for normal fusion of myoblasts to skeletal muscle myotubes. Required for translocation of FER1L5 to the plasma membrane. Binds ATP; does not bind GTP (By similarity).
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 415-449 from the human protein was used as the immunogen for the EHD2 antibody.
Limitation:
This EHD2 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q9NZN4