LC3A Antibody / MAP1LC3A

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NSJ Bioreagents
Product Code: NSJ-F54514
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54514-0.05ML0.05 ml£260.00
Quantity:
NSJ-F54514-0.2ML0.2 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the MAP1LC3A antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
IHC testing of FFPE human heart tissue with MAP1LC3A antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 4
IHC testing of FFPE human brain tissue with MAP1LC3A antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
3 / 4
Western blot testing of 1) human brain, 2) human U-251 MG, 3) mouse NIH 3T3 and 4) mouse brain lysate with MAP1LC3A antibody. Predicted molecular weight ~14 kDa.
4 / 4
Western blot testing of 1) human brain, 2) mouse brain and 3) rat brain lysate with MAP1LC3A antibody. Predicted molecular weight ~14 kDa.
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IHC testing of FFPE human heart tissue with MAP1LC3A antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE human brain tissue with MAP1LC3A antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of 1) human brain, 2) human U-251 MG, 3) mouse NIH 3T3 and 4) mouse brain lysate with MAP1LC3A antibody. Predicted molecular weight ~14 kDa.
Western blot testing of 1) human brain, 2) mouse brain and 3) rat brain lysate with MAP1LC3A antibody. Predicted molecular weight ~14 kDa.

Documents

Further Information

Application Details :
Immunohistochemistry (FFPE): 1:25, Western blot: 1:500-1:2000
Application Note:
The stated application concentrations are suggested starting points. Titration of the MAP1LC3A antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
Recombinant human protein was used as the immunogen for the MAP1LC3A antibody.
Limitation:
This MAP1LC3A antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q9H492