Hemopexin Antibody / HPX

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NSJ Bioreagents
Product Code: NSJ-F54425
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54425-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54425-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the HPX antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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IHC testing of FFPE human liver tissue with HPX antibody. HIER: steam section in pH9 EDTA for 20 min and allow to cool prior to staining.
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IHC testing of FFPE human liver tissue with HPX antibody. HIER: steam section in pH9 EDTA for 20 min and allow to cool prior to staining.
3 / 5
Western blot testing of 1) human HepG2, 2) human plasma, 3) mouse plasma and 4) rat plasma lysate with HPX antibody. Predicted molecular weight: 52-75 kDa depending on glycosylation level.
4 / 5
Western blot testing of 1) human liver and 2) rat liver lysate with HPX antibody. Predicted molecular weight: 52-75 kDa depending on glycosylation level.
5 / 5
Flow cytometry testing of human CCRF-CEM cells with HPX antibody; Blue=isotype control, Green= HPX antibody.
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IHC testing of FFPE human liver tissue with HPX antibody. HIER: steam section in pH9 EDTA for 20 min and allow to cool prior to staining.
IHC testing of FFPE human liver tissue with HPX antibody. HIER: steam section in pH9 EDTA for 20 min and allow to cool prior to staining.
Western blot testing of 1) human HepG2, 2) human plasma, 3) mouse plasma and 4) rat plasma lysate with HPX antibody. Predicted molecular weight: 52-75 kDa depending on glycosylation level.
Western blot testing of 1) human liver and 2) rat liver lysate with HPX antibody. Predicted molecular weight: 52-75 kDa depending on glycosylation level.
Flow cytometry testing of human CCRF-CEM cells with HPX antibody; Blue=isotype control, Green= HPX antibody.

Documents

Further Information

Application Details :
Immunohistochemistry (FFPE): 1:25,Western blot: 1:500-1:2000,Flow cytometry: 1:25 (1x10e6 cells)
Application Note:
The stated application concentrations are suggested starting points. Titration of the HPX antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
HPX is a plasma glycoprotein that binds heme with high affinity. The protein is an acute phase protein that transports heme from the plasma to the liver and may be involved in protecting cells from oxidative stress.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 200-227 from the human protein was used as the immunogen for the HPX antibody.
Limitation:
This HPX antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P02790