PI3KR1 Antibody

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NSJ Bioreagents
Product Code: NSJ-F54451
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54451-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54451-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the PI3KR1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
Western blot testing of human 1) A431, 2) Jurkat, 3) MCF7, 4) K562 and 5) rat brain lysate with PI3KR1 antibody. Predicted molecular weight ~84 kDa.
2 / 4
IHC testing of FFPE human skeletal muscle tissue with PI3KR1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
3 / 4
Immunofluorescent staining of fixed and permeabilized human HeLa cells with PI3KR1 antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
4 / 4
Flow cytometry testing of fixed and permeabilized human HeLa cells with PI3KR1 antibody; Blue=isotype control, Green= PI3KR1 antibody.
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Western blot testing of human 1) A431, 2) Jurkat, 3) MCF7, 4) K562 and 5) rat brain lysate with PI3KR1 antibody. Predicted molecular weight ~84 kDa.
IHC testing of FFPE human skeletal muscle tissue with PI3KR1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of fixed and permeabilized human HeLa cells with PI3KR1 antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
Flow cytometry testing of fixed and permeabilized human HeLa cells with PI3KR1 antibody; Blue=isotype control, Green= PI3KR1 antibody.

Documents

Further Information

Application Details :
Immunofluorescence: 1:25,Western blot: 1:500-1:2000,Flow cytometry: 1:25 (1x10e6 cells),Immunohistochemistry (FFPE): 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the PI3KR1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 1-30 from the human protein was used as the immunogen for the PI3KR1 antibody.
Limitation:
This PI3KR1 antibody is available for research use only.
Localization:
Nuclear, cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human, Rat
Uniprot #:
P27986