PCDH20 Antibody / Protocadherin 20

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NSJ Bioreagents
Product Code: NSJ-F54354
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54354-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54354-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the Protocadherin 20 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 5
Western blot testing of human 1) SK-BR-3, 2) SH-SY5Y, 3) MCF7, 4) HL60 and 5) rat C6 cell lysate with Protocadherin 20 antibody. Predicted molecular weight ~105 kDa.
2 / 5
Western blot testing of human 1) SK-BR-3, 2) SH-SY5Y, 3) MCF7, 4) HL60 and 5) rat brain lysate with Protocadherin 20 antibody. Predicted molecular weight ~105 kDa.
3 / 5
Immunofluorescent staining of fixed and permeabilized human MCF7 cells with Protocadherin 20 antibody (green) and DAPI nuclear stain (blue).
4 / 5
IHC testing of FFPE human pancreas tissue with Protocadherin 20 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
5 / 5
Flow cytometry testing of fixed and permeabilized human MCF7 cells with Protocadherin 20 antibody; Blue=isotype control, Green= Protocadherin 20 antibody.
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Western blot testing of human 1) SK-BR-3, 2) SH-SY5Y, 3) MCF7, 4) HL60 and 5) rat C6 cell lysate with Protocadherin 20 antibody. Predicted molecular weight ~105 kDa.
Western blot testing of human 1) SK-BR-3, 2) SH-SY5Y, 3) MCF7, 4) HL60 and 5) rat brain lysate with Protocadherin 20 antibody. Predicted molecular weight ~105 kDa.
Immunofluorescent staining of fixed and permeabilized human MCF7 cells with Protocadherin 20 antibody (green) and DAPI nuclear stain (blue).
IHC testing of FFPE human pancreas tissue with Protocadherin 20 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Flow cytometry testing of fixed and permeabilized human MCF7 cells with Protocadherin 20 antibody; Blue=isotype control, Green= Protocadherin 20 antibody.

Documents

Further Information

Application Details :
Western blot: 1:500-1:2000,Immunofluorescence: 1:25,Flow cytometry: 1:25 (1x10e6 cells),Immunohistochemistry (FFPE): 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the Protocadherin 20 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This gene belongs to the protocadherin gene family, a subfamily of the cadherin superfamily. This gene encodes a protein which contains 6 extracellular cadherin domains, a transmembrane domain and a cytoplasmic tail differing from those of the classical cadherins. Although its specific function is undetermined, the cadherin-related neuronal receptor is thought to play a role in the establishment and function of specific cell-cell connections in the brain.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 445-472 from the human protein was used as the immunogen for the Protocadherin 20 antibody.
Limitation:
This Protocadherin 20 antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human, Rat
Uniprot #:
Q8N6Y1